Technological watch

“Toehold Switches; a foothold for Synthetic Biology”

ABSTRACTToehold switches are de novo designed riboregulators that contain two RNA components interacting through linear?linear RNA interactions, regulating the gene expression. These are highly versatile, exhibit excellent orthogonality, wide dynamic range, and are highly programmable, so can be used for various applications in synthetic biology. In this review, we summarized and discussed the design characteristics and benefits of toehold switch riboregulators over conventional riboregulators. We also discussed applications and recent advancements of toehold switch riboregulators in various fields like gene editing, DNA nanotechnology, translational repression, and diagnostics (detection of microRNAs and some pathogens). Toehold switches, therefore, furnished advancement in the applications of synthetic biology in various fields by concealing drawbacks with their prominent features.This article is protected by copyright. All rights reserved.

Publication date: 16/12/2022



This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 870292.