Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols
Cyanobacteria Synechocystis sp. PCC 6803 was exploited as green cell factory for light-powered asymmetric synthesis of aromatic chiral alcohols. The effect of temperature, light, substrate and cell concentration on substrate conversions were investigated. Under the optimal condition, a series of chiral alcohols were synthesized with conversions up to 95% and enantiomer excess (ee)?>?99%. We found that the addition of Na2S2O3 and Angeli’s Salt increased the NADPH content by 20% and 25%, respectively. As a result, the time to reach 95% substrate conversion was shortened by 12?h, which demonstrated that the NADPH regeneration and hence the reaction rates can be regulated in cyanobacteria. This blue-green algae based biocatalysis showed its potential for chiral compounds production in future.
Publication date: 01/01/2022
Author: Jianhua Fan, Yinghui Zhang, Ping Wu, Xiaoyan Zhang, Yunpeng Bai