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Cardiac tissue engineering is an emerging approach for cardiac regeneration utilizing the inherent healing responses elicited by the surviving heart using biomaterial templates. In this study, we aimed to develop hydrogel scaffolds for cardiac tissue regeneration following myocardial infarction (MI). Two superabsorbent hydrogels, CAHA2A and CAHA2AP, were developed employing the interpenetration chemistry. CAHA2A was constituted with alginate, carboxymethyl cellulose, (hydroxyethyl) methacrylate (HEMA), and acrylic acid, where CAHA2AP was prepared by interpenetrated CAHA2A with polyvinyl alcohol (PVA). Both hydrogels displayed superior physiochemical characteristics, as determined by AT?IR spectral analysis, DSC measurements, tensile testing, contact angle, water profiling, dye release, and conductivity. In vitro degradation of the hydrogels displayed acceptable weight composure and pH changes. Both hydrogels were hemocompatible, and biocompatible as evident by direct contact and MTT assays. The hydrogels promoted anterograde and retrograde migration as determined by the z?stack analysis using H9c2 cells grown with both gels. Additionally, the co?culture of the hydrogels with swine epicardial adipose tissue (EAT) cells and cardiac fibroblasts resulted in the synchronous growth without any toxicity. Also, both hydrogels facilitated the production of extracellular matrix by the H9c2 cells. Overall, the findings support an appreciable in vitro performance of both hydrogels for cardiac tissue engineering applications.This article is protected by copyright. All rights reserved.