Technological watch

AbstractSericin, a natural protein from silk cocoon, has been reported for various biological properties in the biomaterials field. Modified forms of sericin have been studied for bone tissue engineering, while its unmodified form has been scarcely reported. Therefore, the purpose of this study was to evaluate physical and biological properties of unmodified sericin for potential use in bone surgery. Sericin was extracted from silk cocoons using a chemical-free boiling method. Sericin extract showed distinct bands with molecular weight ranging from 25 to 42 kDa including smear bands. Fourier transform infrared spectra presented characteristic peaks of amide I, II, and III, confirming the chemical composition of sericin. Based on biological activity, sericin extract at a concentration of 40 ?g/mL increased the proliferation of osteoblast cells up to 135%, compared with the untreated control. Moreover, increase in antibacterial activity against Staphylococcus aureus, both clinical isolates and the reference strain ATCC 29213, was demonstrated for sericin extract with normal saline, while no antibacterial activity was observed for sericin with broth. It was found that sericin with normal saline showed higher zeta potential than sericin without normal saline, indicating higher system stability. This was confirmed by the average particle size of sericin extract with NaCl (3,249.3±226.1 nm) showing approximately 10 times smaller than sericin solution (29,015.9 ± 8,085.6 nm). Furthermore, sericin extract at the minimal inhibitory concentration significantly reduced the biofilm formation of S. aureus up to 95%. The study indicates biological activities of sericin, which could be applied as a dual-functional bioactive material to support bone regeneration and treat bone infections.